KMID : 0545120180280020284
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Journal of Microbiology and Biotechnology 2018 Volume.28 No. 2 p.284 ~ p.292
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Biochemical Characterization of a Novel GH86 ¥â-Agarase Producing Neoagarohexaose from Gayadomonas joobiniege G7
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Lee Yeong-Rim
Jung Su-Bin Chi Won-Jae Bae Chang-Hwan Jeong Byeong-Chul Hong Soon-Kwang Lee Chang-Ro
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Abstract
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A novel ¥â-agarase, AgaJ5, was identified from an agar-degrading marine bacterium, Gayadomonas joobiniege G7. It belongs to the glycoside hydrolase family 86 and is composed of 805 amino acids with a 30-amino-acid signal peptide. Zymogram analysis showed that purified AgaJ5 has agarase activity. The optimum temperature and pH for AgaJ5 activity were determined to be 30¡ÆC and 4.5, respectively. AgaJ5 was an acidic ¥â-agarase that had strong activity at a narrow pH range of 4.5-5.5, and was a cold-adapted enzyme, retaining 40% of enzymatic activity at 10¡ÆC. AgaJ5 required monovalent ions such as Na+ and K+ for its maximum activity, but its activity was severely inhibited by several metal ions. The Km and Vmax of AgaJ5 for agarose were 8.9 mg/ml and 188.6 U/mg, respectively. Notably, thin-layer chromatography, mass spectrometry, and agarose-liquefication analyses revealed that AgaJ5 was an endo-type ¥â-agarase producing neoagarohexaose as the final main product of agarose hydrolysis. Therefore, these results suggest that AgaJ5 from G. joobiniege G7 is a novel endotype neoagarohexaose-producing ¥â-agarase having specific biochemical features that may be useful for industrial applications.
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KEYWORD
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agar, ¥â-agarase, GH86, Gayadomonas joobiniege
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